Pressure Cycling-Accelerated Digestion with Trypsin: Protein Modifications and Enzyme Specificity

Pressure Cycling-Accelerated Digestion with Trypsin: Protein Modifications and Enzyme Specificity

Introduction

Here we report that 90 minute pressure-accelerated trypsin digestion of whole tissue lysate is comparable to, or better than, standard overnight digestion. The goal of this work is to provide the user with the best set of starting conditions for pressure-enhanced trypsin digestion of complex samples.