Mitochondria Application Notes

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Introduction
Isolation of intact mitochondria from human and animal tissue is crucial for studies in aging, diabetes and cancer. High quality functional mitochondrial isolates are also important for drug screening studies [1]. Mitochondria isolation from solid tissue is usually performed by labor-intensive homogenizer-based methods [2] that require extensive operator experience. To facilitate efficient and reproducible mitochondria preparation, we have developed a semi-automated method to release mitochondria from solid fibrous tissues, such as muscle, using The PCT Shredder for tissue homogenization. This method can be combined with a subsequent treatment using pressure cycling technology (PCT) to further increase yield, if desired.

Introduction
Isolation of intact mitochondria from human and animal tissue is crucial for studies in aging, diabetes and cancer. High quality functional mitochondrial isolates are also important for drug screening studies [1]. Mitochondria isolation from solid tissue is usually performed by labor-intensive homogenizer-based methods [2] that require extensive operator experience. To facilitate efficient and reproducible mitochondria preparation, we have developed a semi-automated method to release mitochondria from solid fibrous tissues, such as lung, using The PCT Shredder for tissue homogenization. This method can be combined with a subsequent treatment using pressure cycling technology (PCT) to further increase yield, if desired.

Introduction
Isolation of intact mitochondria from human and animal tissue is crucial for studies that focus on the elucidation of their function and dysfunction in conditions such as aging, diabetes and cancer. As potential drug targets, high quality functional mitochondrial isolates are important for drug screening studies [1]. Mitochondria isolation from solid tissue is usually carried out using labor-intensive homogenizer-based methods [2] that require extensive operator experience. Here we describe a semi-automated method to release mitochondria from solid rat brain tissue, using a PBI Shredder and PCT in place of traditional manual homogenization.

Introduction
Isolation of intact mitochondria from human and animal tissue is crucial for studies that focus on the elucidation of their function and dysfunction in conditions such as aging, diabetes and cancer. As potential drug targets, high quality functional mitochondrial isolates are important for drug screening studies [1]. Mitochondria isolation from solid tissue is usually carried out using labor-intensive homogenizer-based methods [2] that require extensive operator experience. Here we describe a semi-automated method to release mitochondria from whole rat heart tissue, using a PBI Shredder and PCT in place of traditional manual homogenization.

Introduction
Proteomic profiling of mitochondria has the potential to provide insights into mitochondrial functions associated with aging, various metabolic states and diseases such as cancer, diabetes and cardiovascular disease [1]. Rapid and reproducible isolation of intact mitochondria is crucial for efficient enrichment and subsequent proteomic analysis of low abundance mitochondrial proteins [2]. Here we describe a system for the isolation of intact mitochondria from rat PC12 cells using pressure cycling technology (PCT).