Introduction
PCT has been proven to accelerate enzymatic protein digestion and the positive effect of PCT on digestion by both trypsin and Lys-C is well established [1-4]. Additionally, the enhancing effects of PCT on the activity of enzymes including Proteinase K, PNGase F, chymotrypsin, and lysozyme have been reported [5-8]. High hydrostatic pressure accelerates protein digestion via a combination of two mechanisms.