Improved Protein Extraction and Protein Identification from Archival Formalin-Fixed Paraffin-Embedded Human Aortas

Conclusions and Clinical Relevance

Combination of heat and high pressure is an effective method to extract proteins from FFPE aorta for downstream proteomics. This method opens the possibility for use of archival and often rare FFPE aortas and possibly other tissues available to proteomics for biomarker discovery and quantification.