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Pressure-Enhanced Enzymes

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Introduction
Pressure Cycling Technology (PCT) has been proven to accelerate enzymatic protein digestion. The positive effect of PCT on trypsin digestion has been demonstrated by several laboratories [1, 2, 3] who have reported improved sequence coverage, higher recovery and significantly reduced digestion times when the trypsin reactions were carried out under pressure. Not only has PCT been shown to accelerate and improve digestion in solution, but it can also accelerate in-gel trypsin digestion [4, 5]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, Lys-C and lysozyme has been reported [6, 7, 8, 9].

Vera Gross; Greta Carlson; Alexander R. Ivanov; Scott Walmsley; Alexey Nesvizhskii; and Alexander Lazarev Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA, USA ; Barnett Institute, Northeastern University, Boston, MA, USA; University of Michigan, 1301 Catherine St., Ann Arbor, MI

Lee B1, Lopez-Ferrer D, Kim BC, Na HB, Park YI, Weitz KK, Warner MG, Hyeon T, Lee SW, Smith RD, Kim J.

Zoltan Szabo, András Guttman and Barry L. Karger*
Barnett Institute, Northeastern University, Boston, Massachusetts 02115