Microflow DIA Using 15 min Gradients Analyzes 40 Tumor Proteomes per Day and Effectively Detects Promising Protein Biomarkers

Microflow DIA Using 15 min Gradients Analyzes 40 Tumor Proteomes per Day and Effectively Detects Promising Protein Biomarkers

The typical gradients used with nanoflow chromatography are 2-3 hours, allowing only 8 complex proteomesThe typical gradients used with nanoflow chromatography are 2-3 hours, allowing only 8 complex proteomessamples to be analyzed in a working day. Increasingly microflow chromatography has been combined withSWATH Acquisition MS to accelerate quantitative proteomics. Very good protein quantitation numbers have beendemonstrated with shortened gradients. In this study, we explore whether using a 15 min microflow LC gradientwith SWATH MS, enabling throughput of 40 proteome samples per day, is robust, practical and effective forprostate cancer tissue biomarker discovery (Figure 1).