Introduction
Proteomic profiling of mitochondria has the potential to provide insights into mitochondrial functions associated with aging, various metabolic states, and diseases such as cancer, diabetes, and cardiovascular disease [1]. Rapid and reproducible isolation of intact mitochondria is crucial for efficient enrichment and subsequent proteomic analysis of low-abundance mitochondrial proteins [2]. Here we describe a system for the isolation of intact mitochondria from rat PC12 cells using pressure cycling technology (PCT).