Pressure-Accelerated Protein Digestion with Endoproteinase Lys-C
Pressure Cycling Technology (PCT) has been proven to accelerate enzymatic protein digestion. The positive effect of PCT on trypsin digestion is well established [1-4] for improved sequence coverage, higher recovery and significantly reduced digestion times. Not only has PCT been shown to accelerate and improve digestion in solution, but it can also accelerate in-gel trypsin digestion [4, 5]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, chymotrypsin and lysozyme has been reported [6-10].