Proteolysis-PrEP (Lysozyme): Effect of Pressure Cycling on Lysozyme Activity

Proteolysis-PrEP (Lysozyme): Effect of Pressure Cycling on Lysozyme Activity

Introduction
Pressure cycling technology (PCT) has been proven to accelerate enzymatic protein digestion. For example, the effect of PCT on trypsin digestion has been demonstrated by several laboratories. They report that digestion times can be reduced from hours to minutes [1, 2]. Not only has PCT been shown to accelerate and improve protein digestion in solution, but it also can accelerate the digestion by trypsin of proteins in polyacrylamide gel slices [3]. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Proteinase K, PNGase F, and Lys-C, has been reported [4, 5, 6]. It is thought that PCT may act synergistically with other protein denaturants, such as organic solvents and elevated temperature, to help maintain substrates in a denatured state leading to more exposure of enzyme target sites which results in better cleavage,. Here we report the enhanced effect by PCT on the activity of the enzyme lysozyme. Lysozyme acts to hydrolyze peptidoglycans found in bacterial cell walls. This enzyme is frequently used for bacterial cell lysis prior to extracting DNA or proteins from bacteria. We also propose an alternate or additional mechanism by which PCT may enhance the activity of lysozyme.