Introduction
The positive effect of Pressure Cycling Technology (PCT) on trypsin digestion is well established [1-4], and has been shown to result in improved sequence coverage, higher recovery and significantly reduced digestion times. Additionally, the enhancing effect of PCT on the activity of several other enzymes, including Lys-C, Proteinase K, PNGase F, chymotrypsin, Glu-C, thermolysin, and lysozyme has been reported [5-12].