Differential Extraction Using Alkaline Lysis and Pressure
We have developed a novel differential extraction method that involves selective digestion of sperm and female epithelial cells using alkaline lysis and pressure cycling
The procedure involves first lysing and removal of female cells via pressure cycling in 0.4N NaOH followed by lysing male cells in 0.4N NaOH at 95°C.
The procedure is rapid (10 min) and results in high recovery of cells directly from cotton swabs
The procedure works well on a variety of substrates and recovers complete male profile for ratios up to 10 to 1.
Future work involves improving selectivity for high female/male ratio