The Signature Exosome Map for Bone Marrow Stromal Cells: Development of an Optimal Sample Preparation Approach Under High Pressure Cycling: Enzyme Specificity, Efficiency and Synergy of Pressure with Acid-Labile Detergents
1. hMSCs produce very little exosomes in the culture medium without FBS compared to that in culture medium with FBS.
2. Exosomes isolated by Total Exosome Isolation kit (Invitrogen) and ExoQuick (SBI)
were with comparable purity and protein yields; while protein extraction of PureExo
(101Bio) -isolated exosomes had lower protein abundance according to 1D SDS-gel
and western blotting.
3. Both barocycler and extraction kit (101Bio) increased protein yield.