Pressure-Enhanced Enzymes Application Notes Display: ASC DESC Sort By: Select Title Date Ordering III. Effect of High Pressure and Temperature on Papainase Digestion Rate INTRODUCTION High hydrostatic pressure (HPP) has been used for decades in the food industry for the inactivation of microbes and of the oxidases responsible for the browning of fruits and vegetables [5]. In biotechnology, HHP has been shown to accelerate the activity of proteases used for the digestion of proteins prior to mass spectrometry. While the positive effects of temperature and pressure on the activity of several proteases are known [6,7], the potential synergy of elevated temperature with pressure has not been fully characterized, particularly in terms of real-time kinetic studies. Using a high pressure optical cell coupled to a programmable high pressure generator, the effects of elevated pressure (0-60,000 psi) and temperature (22°C to 60°C) on the rate at which papainase digests a synthetic substrate was investigated. Download View Details Pressure Cycling-Accelerated Digestion with Trypsin: Protein Modifications and Enzyme Specificity Introduction Here we report that 90 minute pressure-accelerated trypsin digestion of whole tissue lysate is comparable to, or better than, standard overnight digestion. The goal of this work is to provide the user with the best set of starting conditions for pressure-enhanced trypsin digestion of complex samples. Download View Details High Pressure-Accelerated Lys-C Digestion in the HUB880 Explorer: Rapid Digestion of Unreduced IgG Part III. Effects of Urea and Sodium Deoxycholate Introduction The benefit of high pressure incubation for enhanced Lys-C digestion of unreduced IgG, and the added benefit of reagents such as acetonitrile or N-propanol is described in separate Application Notes [4, 5]. In the current Application note we explore the effect of urea and sodium deoxycholate on pressure-enhanced digestion, in order to provide the best set of starting conditions for high pressure-enhanced Lys-C digestion of disulfide-intact IgG in the presence of these reagents. These conditions are likely to be similar for digestion of other hard-to-digest proteins, such as those containing hydrophobic transmembrane domains. The current application focuses on digestion at constant high pressure (not pressure cycling) in the HUB880 Explorer. Download View Details High Pressure-Accelerated Lys-C Digestion in the HUB880 Explorer: Rapid Digestion of Unreduced IgG Part II. Effect of Acetonitrile, N-Propanol, and Methanol Introduction The benefit of high pressure incubation for enhanced Lys-C digestion of unreduced IgG, and the added benefit of reagents such as urea and sodium deoxycholate, is described in separate Application Notes [4, 19]. In the current Application Note we explore the effect of several organic solvents on pressure-enhanced digestion, in order to provide the best set of starting conditions for high pressure-enhanced Lys-C digestion of disulfide-intact IgG in the presence of these reagents. These conditions are likely to be similar for digestion of other hard-to-digest proteins, such as those containing hydrophobic transmembrane domains. The current application focuses on digestion at constant high pressure (not pressure cycling) in the HUB880 Explorer. Download View Details High Pressure-Accelerated Lys-C Digestion in the HUB880 Explorer: Rapid Digestion of Unreduced IgG Part I Introduction The goal of the current Application Note is to provide the best set of starting conditions for high pressure-enhanced Lys-C digestion of disulfide-intact IgG. These conditions are likely to be similar for digestion of other hard-to-digest proteins, such as those containing hydrophobic transmembrane domains. The current application focuses on digestion at constant high pressure (not pressure cycling) in the HUB880 Explorer. Download View Details Start Prev 1 2 3 4 Next End
