Vera Gross¹; Keith Ashman²; Peter Hains³; Alexander Lazarev*,¹; Chris Hodgkins²; Phil Robinson^{3 1}Pressure BioSciences, Inc., 14 Norfolk Ave., South Easton, MA 02375,United States; ²SCIEX, 500 Old Connecticut Path Framingham, MA 01701,United States; ³Children's Medical Research Institute, 214 Hawkesbury Rd., Westmead NSW 2145, Australia

Vera S. Gross1, Greta Carlson1, Emily Freeman2, Alexander R. Ivanov2, Heather Greenberg3, Sergei Baranov3, Irina Stavrovskaya3, Bruce Kristal3, Alexander Lazarev1

1Pressure BioSciences, Inc., South Easton, MA. 2HSPH Proteomics Resource, Harvard School of Public Health, Boston, MA. 3Brigham and Women's Hospital; Harvard University, Boston, MA

Andr�s Guttman1,2, Zolt�n Szab�1 and Barry L. Karger1 1 Barnett Institute, Northeastern University, Boston, MA 02115 2 HLBS, University of Debrecen, Hungary 49th EHPRG, Aug 28-Sept 2, 2011 Budapest, Hungary

Vera Gross¹; John Wilson²; Darryl Pappin²; Alexander Lazarev¹

¹Pressure BioSciences, Inc. Medford, MA; ²Protifi, LLC, Huntington, NY.

E. Bonneil1, R. Biringer2, C. Bell1, P. Thibault1 1Institute for Research in Immunology and Cancer Universit� de Montr�al; 2Thermo Fisher Scientific

Alexander Lazarev Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA

Vera Gross; Alexander V. Lazarev; Nathan P. Lawrence; and Richard T. Schumacher

Pressure BioSciences, Inc., South Easton, MA

Scott R. Kronewitter, Gordon W. Slysz, Daniel Lopez-Ferrer, Matthew E. Monroe, Rui Zhao, Thomas L. Fillmore, Karl K. Weitz, Robert A. Heegel, Daniel J. Orton, Brian L. LaMarche, Kevin L. Crowell, Anuj R. Shah, Carrie D. Nicora, Heather M. Brewer, David G. Camp II, Gordon A. Anderson, Ronald J. Moore, and Richard D. Smith

New Products from Pressure BioSciences for Enhanced Proteomic Research Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375

Tiannan Guo1; Li Li2; Ulrich Wagner3; Qing Zhong3; Christine Fritz3; Christian Fankhauser3; Cedric Poyet3; Rebecca Hartmann3; Andreas Beyer2; Peter Wild3; Ruedi Aebersold1, 4
1Dep Biol,Inst Mol Sys Biol, ETH Zurich, Zurich, SWITZERLAND; 2CECAD, University of Cologne, Cologne, Germany; 3Institute of Surgical Pathology, University Hospital, Zürich, Switzerland; 4Faculty of Science, University of Zurich, Zurich, Switzerland

Alexander Lazarev, Timothy Straub, James Behnke, Edmund Y. Ting Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA

Vera Gross¹; John Wilson²; Alexander Lazarev¹

¹Pressure BioSciences, Inc. Medford, MA; ²Protifi, LLC, Huntington, NY.

P. Pevsner¹, G. Li2, N. Sobol¹, D.C Miller¹, D. Robertson^2,3

¹Department of Pathology and Anatomical Sciences University of Missouri School of Medicine, ²University of Missouri Department of Chemistry 3University of Missouri Research Reactor (MURR) Columbia, MO, USA

Capitalizing on thermodynamic principles to reduce sample preparation times from hours to minutes Daniel L�pez-Ferrer, Biological Separations & Mass Spectrometry Group, Pacific Northwest National Laboratory

Lori C Stansberry; Angela Payne; Julie Skinner; Loren Schultz; Jon Heinrichs; Eberhard Durr Merck and Co., West Point, PA

Alexander Lazarev1; Vera Gross1; Greta Carlson1; Alexander Ivanov2; Scott Walmsley3; Alexey Nesvizhskii3 1 Pressure Bioscience Inc., 14 Norfolk Ave. South Easton, MA 02375 2 Barnett Institute, Northeastern University, 360 Huntington Avenue, Boston MA 02115 3 University of Michigan, 1301 Catherine St., Ann Arbor, MI 48109

Taha Rezai¹, Vera Gross², Alexander Lazarev², Mary Lopez¹

¹Thermo Fisher Scientific, Cambridge, MA; ²Pressure BioSciences Inc., Boston, MA MSACL 2011

Zoltan Szabo, Andris Guttman, Tomas Rejtar and Barry L. Karger Barnett Institute, Boston, MA, USA

Alexander Lazarev*, Vera Gross, Hembly Rivas, James Behnke, Edmund Y. Ting Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375, USA

Yazen Jmeian; Eric Beil; Dariusz Janecki; Steven Pomerantz; Jennifer Nemeth

Janssen Research & Development, Radnor, PA

Abstract Only

Deepthi Nori*, MFS; Dr. Bruce R. McCord, PhD International Forensic Research Institute, Department of Chemistry and Biochemistry Florida International University, Miami, FL USA

1Pressure BioSciences, Inc., South Easton, MA.; 2Harvard Catalyst Laboratory of Innovative Translational Technologies, Boston, MA.;3Brigham and Women's Hospital; Harvard University, Boston, MA.

Rudy Alvarado, Diana Tran, Bonnie Ching, and Brett S. Phinney* UC Davis Proteomics Core Facility, University of California Davis Genome Center, Davis, CA 95616, USA

Louis S. Tisa, Gary B. Smejkal Tom Berkelma, Chiara Rivas-Morello, Alexander Trachtenberg and Winston P. Kuo

A. Lazarev, T. Straub, J. Behnke, E. Y. Ting Pressure BioSciences Inc., Boston, MA, USA

Lorna L. Maheu, Heather M. Connelly, Adam G. Harder, and Steven L. Cockrill Analytical Sciences, Amgen Inc., Longmont, CO 80503

K. L. SCHROEDER, P. A. Okubara, and T. C. Paulitz USDA-ARS, Washington State University, Pullman, WA 99164-6430

Daniel B. Kassel¹; Kheng B. Lim¹; Melinda Manuel¹; Teruaki Okuda²; Naomi Kamiguchi²; Christie L Hunter³; Brian Williamson³; Lydia Nuwaysir^{3 1}Takeda San Diego, Inc, San Diego, CA; ²Takeda Pharmaceutical Company, Limited, Osaka, Japan; ³AB Sciex, Foster City, CA

Emily Freeman and Alexander R. Ivanov Harvard School of Public Health, Department of Genetics and Complex Diseases

New Products from Pressure BioSciences for Enhanced Proteomic Research Pressure BioSciences, Inc. 14 Norfolk Ave., South Easton, MA 02375

Roger Biringer¹, Eric Bonneil², Julian Saba¹, Andreas Huhmer¹, Pierre Thibault²

Johannes Burtscher a, Luca Zangrandi a, Christoph Schwarzer a, Erich Gnaiger b,c, a Dept. Pharmacology, Medical University Innsbruck, Peter-Mayr-Str. 1a, 6020 Innsbruck, Austria b D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, Anichstr. 35, 6020 Innsbruck, Austria c OROBOROS INSTRUMENTS, Schöpfstr. 18, 6020 Innsbruck, Austria Mitochondrion 25 (2015) 104–112

Anna Tomin,a Alexander Lazarev,b Matthew P. Bere,a Hana Redjeba and B�la T�r�k*a Org. Biomol. Chem., 2012, 10, 7321

Deepthi Nori and Bruce R. McCord* Department of Chemistry and Biochemistry Florida International University This email address is being protected from spambots. You need JavaScript enabled to view it.

Melinda A. McFarland ; Denis Andrzejewski; John H. Callahan; Steven M. Musser FDA-CFSAN, College Park, MD

Samuel T Mindaye*, Moonjin Ra*, Jessica Lo Surdo**, Steven Bauer**, Michail Alterman* *Tumor Vaccines and Biotechnology Branch, **Cellular and Tissue Therapies Branch, Division of Cellular and Gene Therapies, CBER Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, Bethesda, Maryland 20892

Seok-Won Hyung¹; Daniel L�pez-Ferrer¹; Daniel J. Orton¹; Erika Zink¹; Karl K. Weitz¹; Rui Zhao¹; Ronald J. Moore¹; Kim K. Hixson¹; Edmund Y. Ting²; Alexander V. Lazarev²; Richard D. Smith¹

¹Pacific Northwest National Laboratory, Richland, WA; ²Pressure Biosciences, Inc, South Easton, MA

Denis Andrzejewski¹; John H. Callahan¹; Timothy Croley¹; Peter E. Leopold²; Melinda A. McFarland¹

¹FDA-CFSAN, College Park , MD; ²BioAnalyte, Inc., Portland, ME ASMS 2013

Gelin, S., Dostie, K., Bresemen, M., Battocletti, A., Thornber, C., Swanson, JD. 5th Annual RI SURF Conference, University of Rhode Island, Kingston RI, July 27, 2012

Steen Larsen a, Regitze Kraunsøe a, Martin Gram a, Erich Gnaiger b, Jørn W. Helge a, Flemming Dela a a Xlab, Center for Healthy Aging, Department of Biomedical Sciences, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark b Department of Visceral, Transplant, and Thoracic Surgery, D. Swarovski Research Laboratory, Medical University of Innsbruck, A-6020 Innsbruck, Austria Anal. Biochem. (2013)

Alexander V. Lazarev¹; Emily Freeman²; Vera S. Gross¹; Greta Carlson1; Edmund Ting¹; Alexander R. Ivanov²

¹Pressure BioSciences, South Easton, MA; ²Harvard School of Public Health, Boston, MA

Michael Lerch, Zhongyu Yang, Evan Brooks, and Wayne L. Hubbell Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA

Pam Marshall University of North Texas Health Science Center, Center for Human Identification

Alexander V. Lazarev¹*; Vera S. Gross¹; Greta Carlson¹; Edmund Ting¹; Emily Freeman²; Alexander R. Ivanov²; Melkamu Getie-Kebtie³; Michail Alterman^3
1Pressure BioSciences, South Easton, MA, USA; ²Harvard School of Public Health, Boston, MA, USA; 3FDA, CBER, Rockville , MD, USA
*Please address all correspondence to This email address is being protected from spambots. You need JavaScript enabled to view it.

John McCoy and Wayne L. Hubbell1 Jules Stein Eye Institute and Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 Contributed by Wayne L. Hubbell, December 6, 2010 (sent for review October 5, 2010) www.pnas.org/cgi/doi/10.1073/pnas.1017877108

Dr. Luke V. Schneider, CSO, Target Discovery, Inc.

Samuel T Mindaye*, Moonjin Ra*, Jessica Lo Surdo**, Steven Bauer**, Michail Alterman* CBER *Tumor Vaccines and Biotechnology Branch, **Cellular and Tissue Therapies Branch, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research Food

Melkamu Getie-Kebtie1; Maryna Eichelberger2, Manju B. Joshi13, Rajesh Gupta3, Michail A. Alterman1 1FDA/CBER/OCTGT/TVBB; 2FDA/CBER/OVRR/LPRVD; 3FDA/CBER/OVRR/PQLS

Li, YF;1, Li, Y;2* Xu, L;1* Servais, J;1 Frank, E;2 Lazarev, A;3 Olsen, BR;1 Grodzinsky, AJ;2 Medici, D;1,4 1Harvard School of Dental Medicine and Harvard Medical School, Boston, MA, 2MIT, Cambridge, MA, 3 Pressure BioSciences, Inc, 4BIDMC, Boston, MA Orthopedic Research Society (ORS) 2011 ORS Annual Meeting

Michael T. Lercha,b, Zhongyu Yanga, Evan K. Brooksa, and Wayne L. Hubbella,b,1 aJules Stein Eye Institute and bDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 PNAS | Published online March 18, 2014 | E1201-E1210

Pam Marshall, MS; Jonathan King, MS; Meredith Turnbough, PhD; Arthur J. Eisenberg, PhD; Bruce Budowle, PhD Institute of Investigative Genetics, Department of Forensics and Investigative Genetics, University of North Texas Health Science Center, Fort Worth, Texas

Daniel L�pez-Ferrer Caprion Proteomics US LLC

Green Mountain Meeting Deepthi Nori and Bruce McCord Department of Chemistry and Biochemistry Florida International University, Miami, FL This email address is being protected from spambots. You need JavaScript enabled to view it.

Mary Thompson, MS, F-ABC, Rebecca Schultheiss Mikulasovich, MS, Mark Powell, MS, F-ABC, Michael Donley, MS, F-ABC, Roger Kahn, PhD, F-ABC Harris County Institute of Forensic Sciences 1885 Old Spanish Trail Houston, TX 77054

Paul H Pevsner et al., University of Missouri School of Medicine

Nicholas Sobol, Jennifer Oprihory*, Tiffany Remsen, A. Yuen, P., Paul Pevsner. Department of Pathology and Anatomical Sciences University of Missouri School of Medicine, Columbia, MO

Gary B. Smejkal1,3, George O. Poinar Jr.2, Feixia Chu3 and Pier Giorgio Righetti4 1 Harvard Catalyst, Harvard Clinical and Translational Science Center, Laboratory for Innovative Translational Technologies, Boston, MA, USA. 2 Oregon State University, Department of Zoology, Corvallis, OR, USA. 3 University of New Hampshire, Hubbard Center for Genome Studies, Durham, NH, USA. 4 Politecnico di Milano, Department of Chemistry, Materials, and Chemical Engineering, Milan, Italy.

Nolwennig Rivalaina,c, Jean Roquainb,c, G�rard Demazeaua,c ICMCB-CNRS � Universit� de Bordeaux � 87, avenue du Dr. Albert Schweitzer, 33608 PESSAC Cedex, France Universit� Victor S�galen Bordeaux 2 � Universit� de Bordeaux � 146, rue L�o Saignat, 33076 Bordeaux Cedex, France Plateforme Technologique d'Innovation Biom�dicale, Avenue du Haut L�v�que, 33600 PESSAC, France

Shiying Shao a,b, Tiannan Guo b, Ruedi Aebersold b,c, a Division of Endocrinology, Tongji Hospital, Huazhong University of Science & Technology, Wuhan 430030, PR China b Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Wolfgang-Pauli-Str. 16, 8093, Switzerland c Faculty of Science, University of Zurich, 8057 Zurich, Switzerland Biochimica et Biophysica Acta 1854 (2015) 519-527

Natalia Pripuzova, Melkamu Getie-Kebtie, Christopher Grunseich, Colin Sweeney, Harry Malech and Michail Alterman Tumor Vaccine and Biotechnology Branch, Division of Cellular and Gene Therapies, Office of Cellular, Tissue and Gene Therapies Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD; Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD; Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.

L.V. Schneider¹, V. Likhte¹, W. Wright¹, F. Chu¹, E. Cambron¹, A. Baldwin1, J. Krakow¹, A. Lazarev², G. Smejkal³

¹Target Discovery, Inc. ²Pressure BioSciences, Inc., ³Harvard LITT

Zhenning Hong,¹Giuseppe Infusini,¹ Lawreen H. Connors,² Martha Skinner,² Catherine E. Costello^1,2

¹Mass Spectrometry Resource and ²Amyloid Treatment and Research Program, Boston University School of Medicine, Boston, MA USA

Harris County Institute of Forensic Sciences 1885 Old Spanish Trail Houston, TX 77054

Li, YF;1§ Li, Y;2* Xu, L;1* Servais, J;1 Frank, E;2 Lazarev, A;3 Olsen, BR;1 Grodzinsky, AJ;2 Medici, D;1,4
1Harvard School of Dental Medicine and Harvard Medical School, Boston, MA, 2MIT, Cambridge, MA, 3 Pressure BioSciences, Inc, 4BIDMC, Boston, MA

Patricia Okubara1, Chunqin Li2, Kurtis Schroeder1, and Nathan Lawrence2
1 USDA ARS, Root Disease & Biological Control Research Unit, Pullman, WA
2 Pressure BioSciences, Inc., West Bridgewater, MA

Bioterrorism remains an ever-present threat to the world. Rapid detection systems are critical to civilian security and to reducing casualties on the battlefield. Most research activities to date in counter-bioterrorism have focused on the development of critical amplification, identification, and detection systems. However, sample preparation is an equally critical aspect of rapid identification and detection of bioterror agents, but the enhancement of such systems lags well behind the development of amplification, identification, and detection systems.